ORK08634

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK08634

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Accession No
Product ID	ORK08634
Clone name	fk00095
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	CLDN1
cDNA sequence	DNA sequence (3426 bp) Predicted protein sequence (247 aa)
HaloTag ORF Clone	FHC08634
Flexi ORF Clone	FXC08634
Description	claudin 1

Features of the cloned cDNA sequence

Description

Length: 3426 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	2562 bp
Genome contig ID	gi89161205r_191406197
PolyA signal sequence (AATAAA,-20)	+--------+--------+----*----+---- GCATTACTCTTTTTCAATAAATTGTTTTTTAATTT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAAAAAAGGATTATTGGCTTGTTTTCTAATCATTTATTGCAAGATTGCCA

Features of the protein sequence

Description

Length: 247 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
O95832	1.5e-73	100.0	Claudin-1; Sene...	Homo sapiens
AAV38916	1.5e-73	100.0	claudin 1 [synt...	synthetic construct
XP_001093143	2.9e-73	99.5	similar to clau...	Macaca mulatta
AAD22962	3.3e-73	99.0	claudin-1 [Homo...	Homo sapiens
AAP97236	1.4e-72	98.5	claudin-2 [Homo...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR006187	118	140	PR01077	Claudin tight junction protein
	IPR003548	141	152	PR01377	Claudin-1
	IPR006187	152	173	PR01077	Claudin tight junction protein
	IPR003548	176	183	PR01377	Claudin-1
	IPR003548	188	195	PR01377	Claudin-1
	IPR006187	194	218	PR01077	Claudin tight junction protein
	IPR003548	229	243	PR01377	Claudin-1
HMMPfam	IPR004031	40	218	PF00822	PMP-22/EMP/MP20 and claudin
ScanRegExp	IPR006187	85	100	PS01346	Claudin tight junction protein

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	40	AGLQLLGFILAFLGWIGAIVSTA	62	PRIMARY	23
	2	118	ALMVVGILLGVIAIFVATVGMKC	140	PRIMARY	23
	3	153	RMAVIGGAIFLLAGLAILVATAW	175	PRIMARY	23
	4	201	LFTGWAAASLCLLGGALLCCSC	222	PRIMARY	22