ORK11473

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK11473

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Accession No
Product ID	ORK11473
Clone name	hk00381
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	SLC11A2
cDNA sequence	DNA sequence (4105 bp) Predicted protein sequence (574 aa)
HaloTag ORF Clone	FHC11473
Flexi ORF Clone	FXC11473
Description	solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2

Features of the cloned cDNA sequence

Description

Length: 4105 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	2331 bp
Genome contig ID	gi89161190r_49566064
PolyA signal sequence (ATTAAA,-23)	+--------+--------+----*----+---- ACTTGGTTTCTAATTAAAAAAAAATTTCTTTTTCC
Flanking genome sequence (99980 - 99931)	----+--------+--------+--------+--------+----* AATTTGGTTTCCTGGGTATTTTTTTAACCTTGAAATAATTTATTAATAGG

Features of the protein sequence

Description

Length: 574 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
EAW58164	99.8	solute carrier ...	Homo sapiens
CAD38517	99.8	divalent metal ...	Homo sapiens
BAA24933	100.0	NRAMP2 [Homo sa...	Homo sapiens
AAI00015	99.8	Solute carrier ...	Homo sapiens
EAW58162	99.4	solute carrier ...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR001046	27	156	PD001861	Natural resistance-associated macrophage protein
FPrintScan	IPR001046	162	188	PR00447	Natural resistance-associated macrophage protein
	IPR001046	192	211	PR00447	Natural resistance-associated macrophage protein
	IPR001046	217	238	PR00447	Natural resistance-associated macrophage protein
	IPR001046	269	292	PR00447	Natural resistance-associated macrophage protein
	IPR001046	396	415	PR00447	Natural resistance-associated macrophage protein
	IPR001046	424	441	PR00447	Natural resistance-associated macrophage protein
	IPR001046	456	475	PR00447	Natural resistance-associated macrophage protein
HMMPfam	IPR001046	103	487	PF01566	Natural resistance-associated macrophage protein
HMMTigr	IPR001046	81	480	TIGR01197	Natural resistance-associated macrophage protein

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	118	LWILLLATLVGLLLQRLAARLGV	140	PRIMARY	23
	2	153	QYPKVPRVILWLMVELAIIGSDM	175	PRIMARY	23
	3	183	IAINLLSVGRIPLWGGVLITIAD	205	PRIMARY	23
	4	220	LEAFFGFLITIMALTFGYEY	239	SECONDARY	20
	5	312	ESCIALFVSFIINVFVVSVFAEA	334	PRIMARY	23
	6	373	GVVLGCYFGPAALYIWAVGILAA	395	PRIMARY	23
	7	425	VVLTRSIAIIPTLLVAVFQDVEH	447	PRIMARY	23
	8	456	NVLQSLQLPFALIPILTFTSLRP	478	SECONDARY	23
	9	489	WRIAGGILVLIICSINMYFVVVY	511	PRIMARY	23
	10	524	AAVVSVAYLGFVFYLGWQCLIAL	546	PRIMARY	23