ORK11507

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK11507

Order Kazusa clone(s) from :

Accession No
Product ID	ORK11507
Clone name	fk00640
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	GLRA2
cDNA sequence	DNA sequence (3186 bp) Predicted protein sequence (480 aa)
HaloTag ORF Clone	FHC11507
Flexi ORF Clone	FXC11507
Description	glycine receptor, alpha 2

Features of the cloned cDNA sequence

Description

Length: 3186 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	1320 bp
Genome contig ID	gi89161218f_14357594
PolyA signal sequence (AATAAA,-20)	+--------+--------+----*----+---- CATTTGTTCAATTTCAATAAAGCTAAGTGTGCCGC
Flanking genome sequence (302260 - 302309)	----+--------+--------+--------+--------+----* AAACTTCTCTAGAGGCTTTCGTTTTCATTTTTGAATTAAAATGTAGATAT

Features of the protein sequence

Description

Length: 480 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
EDL40745	1.2e-203	98.2	glycine recepto...	Mus musculus
P23416	1.6e-199	100.0	Glycine recepto...	Homo sapiens
AAR87843	4e-199	99.5	glycine recepto...	Homo sapiens
XP_548866	5.5e-199	99.7	similar to Glyc...	Canis lupus fam...
XP_857203	1.4e-198	99.3	similar to Glyc...	Canis lupus fam...

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR008129	51	71	PR01675	Glycine receptor alpha2
	IPR006201	121	137	PR00252	Neurotransmitter-gated ion-channel
	IPR006201	154	165	PR00252	Neurotransmitter-gated ion-channel
	IPR006201	200	214	PR00252	Neurotransmitter-gated ion-channel
	IPR006201	276	288	PR00252	Neurotransmitter-gated ion-channel
	IPR006028	285	305	PR00253	Gamma-aminobutyric acid A receptor
	IPR006028	311	332	PR00253	Gamma-aminobutyric acid A receptor
	IPR006028	345	366	PR00253	Gamma-aminobutyric acid A receptor
	IPR008129	384	395	PR01675	Glycine receptor alpha2
	IPR008129	416	426	PR01675	Glycine receptor alpha2
	IPR006028	449	469	PR00253	Gamma-aminobutyric acid A receptor
HMMPfam	IPR006202	72	282	PF02931	Neurotransmitter-gated ion-channel ligand-binding
HMMPfam	IPR006029	289	466	PF02932	Neurotransmitter-gated ion-channel transmembrane region
HMMTigr	IPR006201	37	469	TIGR00860	Neurotransmitter-gated ion-channel
ScanRegExp	IPR006201	200	214	PS00236	Neurotransmitter-gated ion-channel

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	29	MNRQLVNILTALFAFFLETNHFR	51	SECONDARY	23
	2	283	GYYLIQMYIPSLLIVILSWVSFW	305	PRIMARY	23
	3	346	DIWMAVCLLFVFAALLEYAAVNF	368	PRIMARY	23