ORK11519

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK11519

Order Kazusa clone(s) from :

Accession No
Product ID	ORK11519
Clone name	fh13102
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	KCNC1
cDNA sequence	DNA sequence (5017 bp) Predicted protein sequence (515 aa)
HaloTag ORF Clone	FHC11519
Flexi ORF Clone	FXC11519
Description	potassium voltage-gated channel, Shaw-related subfamily, member 1

Features of the cloned cDNA sequence

Description

Length: 5017 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	3437 bp
Genome contig ID	gi51511727f_17614082
PolyA signal sequence (None)	+--------+--------+----*----+---- GGGGGGGCGGGAGGGATGCCCTCAGAACCCAGCGT
Flanking genome sequence	None

Features of the protein sequence

Description

Length: 515 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
P48547	100.0	Potassium volta...	Homo sapiens
P15388	99.8	Potassium volta...	Mus musculus
AAI07130	99.8	Potassium volta...	Homo sapiens
XP_001087790	99.6	similar to Pota...	Macaca mulatta
AAX23601	99.4	voltage-gated p...	Bos taurus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR003968	58	68	PR01491	Kv channel
	IPR003091	58	77	PR00169	Voltage-dependent potassium channel
	IPR003974	59	72	PR01498	Shaw voltage-gated K+ channel
	IPR003974	74	89	PR01498	Shaw voltage-gated K+ channel
	IPR003974	92	113	PR01498	Shaw voltage-gated K+ channel
	IPR005403	142	150	PR01581	Kv3.1 voltage-gated K+ channel
	IPR003974	184	196	PR01498	Shaw voltage-gated K+ channel
	IPR003091	187	215	PR00169	Voltage-dependent potassium channel
	IPR005403	216	231	PR01581	Kv3.1 voltage-gated K+ channel
	IPR005403	232	244	PR01581	Kv3.1 voltage-gated K+ channel
	IPR003091	251	274	PR00169	Voltage-dependent potassium channel
	IPR003091	277	297	PR00169	Voltage-dependent potassium channel
	IPR003091	319	345	PR00169	Voltage-dependent potassium channel
	IPR003968	324	332	PR01491	Kv channel
	IPR003968	348	362	PR01491	Kv channel
	IPR003091	348	371	PR00169	Voltage-dependent potassium channel
	IPR003091	388	410	PR00169	Voltage-dependent potassium channel
	IPR003091	417	443	PR00169	Voltage-dependent potassium channel
	IPR003968	428	439	PR01491	Kv channel
	IPR003974	453	467	PR01498	Shaw voltage-gated K+ channel
	IPR005403	479	493	PR01581	Kv3.1 voltage-gated K+ channel
HMMPfam	IPR003131	14	108	PF02214	K+ channel tetramerisation
HMMPfam	IPR005821	251	439	PF00520	Ion transport
HMMSmart	IPR000210	12	116	SM00225	BTB/POZ-like

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	193	ARYVAFASLFFILVSITTFCLET	215	PRIMARY	23
	2	247	AFLTYIEGVCVVWFTFEFLMRVI	269	SECONDARY	23
	3	278	IKNSLNIIDFVAILPFYLEVGLS	300	SECONDARY	23
	4	342	LRASTNEFLLLIIFLALGVLIFA	364	PRIMARY	23
	5	424	CALAGVLTIAMPVPVIVNNFGMY	446	SECONDARY	23