ORK11649

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK11649

Order Kazusa clone(s) from :

Accession No
Product ID	ORK11649
Clone name	pj00267
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	RPAP1
cDNA sequence	DNA sequence (4642 bp) Predicted protein sequence (1415 aa)
HaloTag ORF Clone	FHC11649
Flexi ORF Clone	FXC11649
Description	RNA polymerase II associated protein 1

Features of the cloned cDNA sequence

Description

Length: 4642 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	362 bp
Genome contig ID	gi51511731r_39496670
PolyA signal sequence (AATAAA,-22)	+--------+--------+----*----+---- AGTTGTGCATTAAAATAAATGTTTTTTATCTCCTG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* GAAATTTGATCTGGACTGATTTCAGCCATGTTAGTTCAGGGTTAGTATCT

Features of the protein sequence

Description

Length: 1415 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
EAW92504	99.9	RNA polymerase ...	Homo sapiens
NP_056355	99.8	RNA polymerase ...	Homo sapiens
Q9BWH6	99.8	RNA polymerase ...	Homo sapiens
XP_510325	99.4	RNA polymerase ...	Pan troglodytes
XP_001101155	95.9	similar to RNA ...	Macaca mulatta

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR013930	246	294	PF08621	RNA polymerase II-associated protein 1
HMMPfam	IPR013929	376	447	PF08620	RNA polymerase II-associated protein 1

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	698	RLWAVAASYGQGGYLYRELYPVL	720	SECONDARY	23
	2	743	RIASLLTLLTQLTLAAGSTPAET	765	SECONDARY	23
	3	772	ASLSATPSLVTWTQVSGLQPLVE	794	SECONDARY	23
	4	805	SRPEMWRAVGPVPVACLLFLGA	826	PRIMARY	22
	5	916	LTALLSLLNTLAQIHKGLCGQLA	938	SECONDARY	23