ORK05473

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK05473

Order Kazusa clone(s) from :

Accession No	AB209506
Product ID	ORK05473
Clone name	ff09358
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	HTT
cDNA sequence	DNA sequence (11155 bp) Predicted protein sequence (1147 aa)
Description	Huntingtin (Huntington disease protein) (HD protein).

Features of the cloned cDNA sequence

Description

Length: 11155 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : YES

Integrity of 3' end

Length of 3'UTR	5806 bp
Genome contig ID	gi89161207f_2999864
PolyA signal sequence (AGTAAA,-23)	+--------+--------+----*----+---- TTTAATTATATCAGTAAAGAGATTAATTTTAACGT
Flanking genome sequence (212320 - 212369)	----+--------+--------+--------+--------+----* AACTCTTTCTATGCCCGTGTAAAGTATGTGAATCGCAAGGCCTGTGCTGC

Features of the protein sequence

Description

Length: 1147 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAD92743	100.0	huntingtin vari...	Homo sapiens
EAW82474	99.6	huntingtin (Hun...	Homo sapiens
EAW82475	99.6	huntingtin (Hun...	Homo sapiens
EAW82478	99.6	huntingtin (Hun...	Homo sapiens
P42858	99.6	Huntingtin; Hun...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR000357	299	336	PF02985	HEAT

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	420	TFGCCEALCLLSTAFPVCIWSLG	442	PRIMARY	23
	2	459	SCTVGMATMILTLLSSAWFPL	479	SECONDARY	21
	3	534	PMVEQLFSHLLKVINICAHVLDD	556	SECONDARY	23
	4	884	QFRESEAIIPNIFFFLVLLS	903	PRIMARY	20
	5	1064	VSTVQLWISGILAILRVLISQST	1086	PRIMARY	23