ORK04563

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK04563

Order Kazusa clone(s) from :

Accession No	AB209359
Product ID	ORK04563
Clone name	fh16154
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector. This cDNA clone fh16154 has an irregular adapter sequence (GTCGACCCAC GCGTCCGGAC CCACGCGTCC G) instead of regular one (GTCGACCCAC GCGTCCG).
Symbol	CHRNA4
cDNA sequence	DNA sequence (5201 bp) Predicted protein sequence (533 aa)
Description	Neuronal acetylcholine receptor subunit alpha-4 precursor.

Features of the cloned cDNA sequence

Description

Length: 5201 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	2669 bp
Genome contig ID	gi51511747r_61346140
PolyA signal sequence (AATAAA,-27)	+--------+--------+----*----+---- TCTGTCTGAATAAACTAAGACGCAGTTTGTCCATC
Flanking genome sequence (99725 - 99676)	----+--------+--------+--------+--------+----* ACCTGTTAGTGGACTTTGCTTGCAGCTGTCGGTGATTAGGAATGGAGGTG

Features of the protein sequence

Description

Length: 533 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAD92596	5.9e-176	100.0	cholinergic rec...	Homo sapiens
AAH96291	1.6e-164	96.7	CHRNA4 protein ...	Homo sapiens
EAW75269	3.8e-163	99.4	cholinergic rec...	Homo sapiens
CAA60959	4e-163	99.4	nicotinic acety...	Homo sapiens
AAH96290	4.1e-163	99.4	Cholinergic rec...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR006201	67	81	PR00252	Neurotransmitter-gated ion-channel
FPrintScan	IPR006201	143	155	PR00252	Neurotransmitter-gated ion-channel
HMMPfam	IPR006202	34	149	PF02931	Neurotransmitter-gated ion-channel ligand-binding
HMMPfam	IPR006029	156	524	PF02932	Neurotransmitter-gated ion-channel transmembrane region
ScanRegExp	IPR006201	67	81	PS00236	Neurotransmitter-gated ion-channel

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	12	RAHPGCACAELSLLWLCCCTFT	33	SECONDARY	22
	2	148	LPLFYTINLIIPCLLISCLTVLV	170	PRIMARY	23
	3	198	ITEIIPSTSLVIPLIGEYLLFTM	220	PRIMARY	23
	4	504	IDRIFLWMFIIVCLLGTVGLFLP	526	PRIMARY	23