ORK10410

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK10410

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Accession No
Product ID	ORK10410
Clone name	fh17222s1
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	RNF213
cDNA sequence	DNA sequence (13625 bp) Predicted protein sequence (4161 aa)
Description	RING finger protein 213

Features of the cloned cDNA sequence

Description

Length: 13625 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	1139 bp
Genome contig ID	gi51511734f_75813539
PolyA signal sequence (None)	+--------+--------+----*----+---- GAGGCAGTATCCTTAATATTCATTCTAAATGAGTT
Flanking genome sequence (169495 - 169544)	----+--------+--------+--------+--------+----* AACGACTTAACTTGAAATTGGGCCTAAGGAGTGAGAACTACAAAAATACA

Features of the protein sequence

Description

Length: 4161 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q63HN8	99.9	RING finger pro...	Homo sapiens
XP_540474	72.2	similar to chro...	Canis lupus fam...
CAH56189	99.9	hypothetical pr...	Homo sapiens
XP_590465	73.0	similar to mCG1...	Bos taurus
EDL34702	70.1	mCG142721, isof...	Mus musculus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR001841	2951	2989	PF00097	Zinc finger
HMMSmart	IPR003593	1366	1511	SM00382	AAA+ ATPase
	IPR003593	1715	1864	SM00382	AAA+ ATPase
	IPR001841	2951	2989	SM00184	Zinc finger
ProfileScan	IPR001841	2951	2989	PS50089	Zinc finger

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	35	LIAVADSVLTKVVGDLLSGTILV	57	SECONDARY	23
SOSUI2	2	1015	SVQTGIWVFLFKLLILQYLMDIN	1037	PRIMARY	23