ORK11622

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK11622

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Accession No
Product ID	ORK11622
Clone name	fj04875
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	HMGCR
cDNA sequence	DNA sequence (4532 bp) Predicted protein sequence (926 aa)
HaloTag ORF Clone	FHC11622
Flexi ORF Clone	FXC11622
Description	3-hydroxy-3-methylglutaryl-Coenzyme A reductase

Features of the cloned cDNA sequence

Description

Length: 4532 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	1751 bp
Genome contig ID	gi51511721f_74568791
PolyA signal sequence (AGTAAA,-22)	+--------+--------+----*----+---- TTTTTCTAGTTTCAGTAAATAATGAAAAGGAAGTT
Flanking genome sequence (124885 - 124934)	----+--------+--------+--------+--------+----* AATACCAATAAGTGTAAGCAGTAACCTTTTTTCTTTGGATTAACCCTAGC

Features of the protein sequence

Description

Length: 926 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
P04035	100.0	3-hydroxy-3-met...	Homo sapiens
BAF85581	99.8	unnamed protein...	Homo sapiens
XP_001148324	99.7	3-hydroxy-3-met...	Pan troglodytes
BAG35346	99.7	unnamed protein...	Homo sapiens
Q5R6N3	99.3	3-hydroxy-3-met...	Pongo abelii

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR002202	564	585	PR00071	Hydroxymethylglutaryl-coenzyme A reductase
	IPR002202	591	611	PR00071	Hydroxymethylglutaryl-coenzyme A reductase
	IPR002202	682	700	PR00071	Hydroxymethylglutaryl-coenzyme A reductase
	IPR002202	722	747	PR00071	Hydroxymethylglutaryl-coenzyme A reductase
	IPR002202	773	799	PR00071	Hydroxymethylglutaryl-coenzyme A reductase
	IPR002202	828	849	PR00071	Hydroxymethylglutaryl-coenzyme A reductase
HMMPfam	IPR002202	513	909	PF00368	Hydroxymethylglutaryl-coenzyme A reductase
HMMTigr	IPR004816	39	926	TIGR00920	Hydroxymethylglutaryl-CoA reductase (NADPH)
HMMTigr	IPR004554	500	909	TIGR00533	3-hydroxy-3-methylglutaryl Coenzyme A reductase
ProfileScan	IPR000731	99	256	PS50156	Sterol-sensing 5TM box
ProfileScan	IPR002202	502	909	PS50065	Hydroxymethylglutaryl-coenzyme A reductase
ScanRegExp	IPR002202	684	698	PS00066	Hydroxymethylglutaryl-coenzyme A reductase
	IPR002202	840	847	PS00318	Hydroxymethylglutaryl-coenzyme A reductase
	IPR002202	894	907	PS01192	Hydroxymethylglutaryl-coenzyme A reductase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	55	PWEVIVGTVTLTICMMSMNMFT	76	SECONDARY	22
	2	96	LSSDIIILTITRCIAILYIYFQF	118	PRIMARY	23
	3	126	SKYILGIAGLFTIFSSFVFSTVV	148	PRIMARY	23
	4	198	GMAILGPTFTLDALVECLVIGVG	220	SECONDARY	23
	5	234	FGCMSVLANYFVFMTFFPACVSL	256	PRIMARY	23
	6	355	SMDIEQVITLSLALLLAVKYIFF	377	PRIMARY	23