ORK06890

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK06890

Order Kazusa clone(s) from :

Accession No	AB209203
Product ID	ORK06890
Clone name	fj06364
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	SLC9A5
cDNA sequence	DNA sequence (4617 bp) Predicted protein sequence (394 aa)
Description	Sodium/hydrogen exchanger 5 (Na(+)/H(+) exchanger 5) (NHE-5) (Solute carrier family 9 member 5).

Features of the cloned cDNA sequence

Description

Length: 4617 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : YES

Integrity of 3' end

Length of 3'UTR	1850 bp
Genome contig ID	gi51511732f_65729087
PolyA signal sequence (ATTAAA,-20)	+--------+--------+----*----+---- TTGAACCTAAGATATATTAAACATCTCTCAGATGG
Flanking genome sequence (134509 - 134558)	----+--------+--------+--------+--------+----* ATGGGTGTCTTGTGTCCATGTGTCAGGGGGTTCTTAGGGTGGGGGGCAAC

Features of the protein sequence

Description

Length: 394 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAD92440	6.7e-162	100.0	solute carrier ...	Homo sapiens
EAW83116	1.1e-149	99.4	solute carrier ...	Homo sapiens
EAW83115	1.3e-149	99.4	solute carrier ...	Homo sapiens
Q14940	1.4e-149	99.4	Sodium/hydrogen...	Homo sapiens
XP_001251992	6e-149	98.9	similar to solu...	Bos taurus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001118	23	30	PR01087	Na+/H+ exchanger
	IPR001118	64	76	PR01087	Na+/H+ exchanger
	IPR001118	224	234	PR01087	Na+/H+ exchanger
	IPR001118	236	245	PR01087	Na+/H+ exchanger
	IPR001118	278	293	PR01087	Na+/H+ exchanger
	IPR001118	294	312	PR01087	Na+/H+ exchanger
HMMPfam	IPR006153	7	217	PF00999	Sodium/hydrogen exchanger
HMMTigr	IPR004709	2	390	TIGR00840	Sodium/hydrogen exchanger subfamily

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	8	SLFVVSLGGAAVGLVFAFLLALT	30	SECONDARY	23
	2	46	FLLAYAAYLTAEMASLSAILAVT	68	PRIMARY	23
	3	93	TMKTLASCAETVIFMLLGISAVD	115	SECONDARY	23
	4	125	LVLGTLIFILFFRALGVVLQTWV	147	PRIMARY	23
	5	179	ILLDRTKVPAKDYFVATTIVVVF	201	PRIMARY	23