ORK05573

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK05573

Order Kazusa clone(s) from :

Accession No	AB208890
Product ID	ORK05573
Clone name	fj13057
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	KCNQ3
cDNA sequence	DNA sequence (3464 bp) Predicted protein sequence (443 aa)
Description	Potassium voltage-gated channel subfamily KQT member 3 (Voltage-gated potassium channel subunit Kv7.3) (Potassium channel subunit alpha KvLQT3) (KQT-like 3).

Features of the cloned cDNA sequence

Description

Length: 3464 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : YES

Integrity of 3' end

Length of 3'UTR	2132 bp
Genome contig ID	gi51511724r_133110574
PolyA signal sequence (None)	+--------+--------+----*----+---- GGGGACACCAATGGCTGCATCAAATGCATGCGTGT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* GCGTGGTGGCCCCACCCAGGCAGGGGCTTCCCACAGCCTCTTCCTCCCCA

Features of the protein sequence

Description

Length: 443 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAD92127	2.1e-181	100.0	potassium volta...	Homo sapiens
AAB97314	1.5e-167	98.3	potassium chann...	Homo sapiens
AAI28577	1.5e-167	98.3	KCNQ3 protein [...	Homo sapiens
XP_001134760	1.5e-167	98.3	potassium volta...	Pan troglodytes
O43525	1.5e-167	98.3	Potassium volta...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR003948	9	22	PR01462	KCNQ3 voltage-gated potassium channel
	IPR003948	60	71	PR01462	KCNQ3 voltage-gated potassium channel
	IPR003091	84	107	PR00169	Voltage-dependent potassium channel
	IPR003937	92	111	PR01459	KCNQ voltage-gated potassium channel
	IPR003937	112	131	PR01459	KCNQ voltage-gated potassium channel
	IPR003948	135	144	PR01462	KCNQ3 voltage-gated potassium channel
	IPR003091	157	183	PR00169	Voltage-dependent potassium channel
	IPR003937	173	192	PR01459	KCNQ voltage-gated potassium channel
	IPR003091	186	209	PR00169	Voltage-dependent potassium channel
	IPR003948	212	225	PR01462	KCNQ3 voltage-gated potassium channel
	IPR003091	226	248	PR00169	Voltage-dependent potassium channel
	IPR003091	255	281	PR00169	Voltage-dependent potassium channel
	IPR003948	389	401	PR01462	KCNQ3 voltage-gated potassium channel
HMMPfam	IPR005821	84	277	PF00520	Ion transport

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	49	LLYHALVFLIVLGCLILAVLTTF	71	PRIMARY	23
	2	82	LLLLETFAIFIFGAEFALRIWAA	104	SECONDARY	23
	3	123	PLCMLDIFVLIASVPVVAVGNQG	145	PRIMARY	23
	4	189	TAWYIGFLTLILSSFLVYLVEKD	211	PRIMARY	23
	5	226	FETYADALWWGLITLATIGYGDK	248	SECONDARY	23
	6	259	AATFSLIGVSFFALPAGILGSGL	281	SECONDARY	23