ORK04212

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK04212

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Accession No	AB208896
Product ID	ORK04212
Clone name	fk10614
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	ATP2B1
cDNA sequence	DNA sequence (3469 bp) Predicted protein sequence (840 aa)
Description	Plasma membrane calcium-transporting ATPase 1 (EC 3.6.3.8) (PMCA1) (Plasma membrane calcium pump isoform 1) (Plasma membrane calcium ATPase isoform 1).

Features of the cloned cDNA sequence

Description

Length: 3469 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : YES

Integrity of 3' end

Length of 3'UTR	840 bp
Genome contig ID	gi89161190r_88408338
PolyA signal sequence (None)	+--------+--------+----*----+---- AAAATTAGTCCTTCAAATACTGCTGTATTTTCAGG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAAAAAAAAAAAAGGGGTGTTTCTATTGAAACTGTACTAAATTTTTGCCT

Features of the protein sequence

Description

Length: 840 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAD92133	100.0	plasma membrane...	Homo sapiens
AAD09925	99.0	plasma membrane...	Homo sapiens
EAW97434	99.0	ATPase, Ca++ tr...	Homo sapiens
XP_865557	98.7	similar to plas...	Canis lupus fam...
XP_865573	98.7	similar to plas...	Canis lupus fam...

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001757	137	151	PR00119	ATPase
	IPR001757	342	353	PR00119	ATPase
	IPR001757	364	374	PR00119	ATPase
	IPR001757	459	478	PR00119	ATPase
	IPR001757	483	495	PR00119	ATPase
HMMPfam	IPR008250	21	129	PF00122	E1-E2 ATPase-associated region
	IPR005834	133	482	PF00702	Haloacid dehalogenase-like hydrolase
	IPR006068	578	729	PF00689	ATPase
HMMTigr	IPR006408	1	728	TIGR01517	Calcium-translocating P-type ATPase
	IPR001757	73	159	TIGR01494	ATPase
	IPR001757	339	389	TIGR01494	ATPase
	IPR001757	426	541	TIGR01494	ATPase
ScanRegExp	IPR001757	139	145	PS00154	ATPase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	37	IGKAGLLMSAITVIILVLYFVID	59	PRIMARY	23
	2	85	IIGVTVLVVAVPEGLPLAVTISL	107	PRIMARY	23
	3	521	QFQLTVNVVAVIVAFTGACITQD	543	PRIMARY	23
	4	599	HAFYQLVVVFTLLFAGEKFFDID	621	SECONDARY	23
	5	634	EHYTIVFNTFVLMQLFNEINA	654	SECONDARY	21
	6	670	NAIFCTIVLGTFVVQIIIVQFGG	692	SECONDARY	23
	7	704	QWLWSIFLGMGTLLWGQLISTIP	726	PRIMARY	23