ORK11825

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK11825

Order Kazusa clone(s) from :

Accession No
Product ID	ORK11825
Clone name	ha06816
Vector information	The cDNA fragment was inserted at the NotI-SalI site of the ... The cDNA fragment was inserted at the NotI-SalI site of the pSPORT1 vector (GenBank Accession No. U12390, AmpR). The 5' end of the cDNA was ligated at the NotI site and the 3' end of the cDNA was inserted at the SalI site of the vector. The adjacent sequence to the 3' end of the cDNA is as follows: GTCGACCCAC GCGTCCGCCC ACGCGTCCGC CCACGCGTCC G-3' end of cDNA.
Symbol	ATP2A2
cDNA sequence	DNA sequence (4694 bp) Predicted protein sequence (1084 aa)
Description	Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (SERCA2)(EC 3.6.3.8)(Calcium pump 2)(Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform)(SR Ca(2+)-ATPase 2)(Endoplasmic reticulum class 1/2 Ca(2+) ATPase)

Features of the cloned cDNA sequence

Description

Length: 4694 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	1438 bp
Genome contig ID	gi89161190f_109103851
PolyA signal sequence (None)	+--------+--------+----*----+---- CAACACATCAGTCATGCACAGGGTTGGTAGATGTG
Flanking genome sequence	None

Features of the protein sequence

Description

Length: 1084 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
P16615	100.0	Sarcoplasmic/en...	Homo sapiens
XP_001141715	99.5	ATPase, Ca++ tr...	Pan troglodytes
P11607	99.0	Sarcoplasmic/en...	Sus scrofa
CAJ42045	99.0	sarcoplasmic/en...	Equus caballus
P11507	98.9	Sarcoplasmic/en...	Rattus norvegicus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001757	218	232	PR00119	ATPase
	IPR001757	391	405	PR00119	ATPase
	IPR001757	636	647	PR00119	ATPase
	IPR001757	658	668	PR00119	ATPase
	IPR000695	714	730	PR00120	H+ transporting ATPase
	IPR000695	742	758	PR00120	H+ transporting ATPase
	IPR001757	742	761	PR00119	ATPase
	IPR001757	765	777	PR00119	ATPase
	IPR000695	773	798	PR00120	H+ transporting ATPase
HMMPfam	IPR004014	41	119	PF00690	ATPase
	IPR008250	135	383	PF00122	E1-E2 ATPase-associated region
	IPR005834	387	765	PF00702	Haloacid dehalogenase-like hydrolase
	IPR006068	860	1032	PF00689	ATPase
HMMTigr	IPR005782	95	1030	TIGR01116	Calcium ATPase
	IPR001757	135	235	TIGR01494	ATPase
	IPR001757	245	410	TIGR01494	ATPase
	IPR001757	633	683	TIGR01494	ATPase
	IPR001757	717	822	TIGR01494	ATPase
ScanRegExp	IPR001757	393	399	PS00154	ATPase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	100	EDLLVRILLLAACISFVLAWFEE	122	PRIMARY	23
	2	128	TAFVEPFVILLILVANAIVGVWQ	150	PRIMARY	23
	3	300	EQLSKVISLICIAVWIINIGHFN	322	PRIMARY	23
	4	341	AVALAVAAIPEGLPAVITTCLAL	363	PRIMARY	23
	5	817	FLTAALGFPEALIPVQLLWVNLV	839	SECONDARY	23
	6	873	WLFFRYLAIGCYVGAATVGAAAW	895	PRIMARY	23
	7	937	PMTMALSVLVTIEMCNALNSLSE	959	SECONDARY	23
	8	975	VGSICLSMSLHFLILYVEPLPLI	997	PRIMARY	23
	9	1003	LNVTQWLMVLKISLPVILMDETL	1025	SECONDARY	23
	10	1055	GISWPFVLLIMPLVIWVYSTDTN	1077	PRIMARY	23