ORK07674

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK07674

Order Kazusa clone(s) from :

Accession No	AB208999
Product ID	ORK07674
Clone name	hk10125
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	GRIN1
cDNA sequence	DNA sequence (4465 bp) Predicted protein sequence (663 aa)
Description	Homo sapiens mRNA for NMDA receptor 1 isoform NR1-2 precursor variant protein.

Features of the cloned cDNA sequence

Description

Length: 4465 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : YES

Integrity of 3' end

Length of 3'UTR	1377 bp
Genome contig ID	gi89161216f_139053490
PolyA signal sequence (None)	+--------+--------+----*----+---- GCTCCTGTGGCCCACTCTGCCCCTGTCTCCCTGTG
Flanking genome sequence (126207 - 126256)	----+--------+--------+--------+--------+----* GCGGCCGCTCTGCCCAGCCCGCCCATGCTGCTCTCTCTCACTCTCTGGAC

Features of the protein sequence

Description

Length: 663 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAD92236	100.0	NMDA receptor 1...	Homo sapiens
CAM17671	99.8	glutamate recep...	Homo sapiens
BAD92155	99.8	NMDA receptor 1...	Homo sapiens
EAW88358	95.8	glutamate recep...	Homo sapiens
EAW88352	95.8	glutamate recep...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	NULL	379	448	PD245531	NULL
FPrintScan	IPR001508	226	254	PR00177	NMDA receptor
	IPR001508	316	341	PR00177	NMDA receptor
	IPR001508	354	378	PR00177	NMDA receptor
	IPR001508	385	412	PR00177	NMDA receptor
	IPR001508	567	591	PR00177	NMDA receptor
HMMPfam	IPR001828	32	112	PF01094	Extracellular ligand-binding receptor
HMMPfam	IPR001320	313	588	PF00060	Ionotropic glutamate receptor
HMMSmart	IPR001320	187	549	SM00079	Ionotropic glutamate receptor

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	314	STLWLLVGLSVHVVAVMLYLLDR	336	PRIMARY	23
	2	355	LTLSSAMWFSWGVLLNSGIGEGA	377	SECONDARY	23
	3	387	GMVWAGFAMIIVASYTANLAAFL	409	PRIMARY	23
	4	569	GVFMLVAGGIVAGIFLIFIEIA	590	PRIMARY	22