ORK06879

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK06879

Order Kazusa clone(s) from :

Accession No	AK226164
Product ID	ORK06879
Clone name	pf04944
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	SLC4A4
cDNA sequence	DNA sequence (7347 bp) Predicted protein sequence (1042 aa)
Description	Electrogenic sodium bicarbonate cotransporter 1 (Sodium bicarbonate cotransporter) (Na(+)/HCO3(-) cotransporter) (Solute carrier family 4 member 4) (kNBC1).

Features of the cloned cDNA sequence

Description

Length: 7347 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	4217 bp
Genome contig ID	gi89161207f_72239838
PolyA signal sequence (AATAAA,-18)	+--------+--------+----*----+---- TTATATGTATACTTAAAAATAAAGTAACTTTATGC
Flanking genome sequence (416827 - 416876)	----+--------+--------+--------+--------+----* ATTTATGACTGTCAAGTGTTTCTGTTCAACTCCTCAAATAATTGTTAAAT

Features of the protein sequence

Description

Length: 1042 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q9Y6R1	100.0	Electrogenic so...	Homo sapiens
AAD42020	99.9	sodium bicarbon...	Homo sapiens
AAG47773	99.8	sodium bicarbon...	Homo sapiens
XP_517243	99.8	solute carrier ...	Pan troglodytes
AAF21718	99.4	electrogenic Na...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR003024	101	107	PR01232	Na+/HCO3- transporter
	IPR003020	281	293	PR01231	HCO3- transporter
	IPR003024	291	299	PR01232	Na+/HCO3- transporter
	IPR003024	332	340	PR01232	Na+/HCO3- transporter
	IPR003024	342	350	PR01232	Na+/HCO3- transporter
	IPR003024	352	364	PR01232	Na+/HCO3- transporter
	IPR003020	422	431	PR01231	HCO3- transporter
	IPR003020	447	458	PR01231	HCO3- transporter
	IPR003024	486	493	PR01232	Na+/HCO3- transporter
	IPR003024	533	540	PR01232	Na+/HCO3- transporter
	IPR003020	537	549	PR01231	HCO3- transporter
	IPR003020	558	570	PR01231	HCO3- transporter
	IPR003020	692	701	PR01231	HCO3- transporter
	IPR003020	756	765	PR01231	HCO3- transporter
	IPR003020	770	783	PR01231	HCO3- transporter
HMMPfam	IPR013769	100	351	PF07565	HCO3- transporter
HMMPfam	IPR011531	398	919	PF00955	HCO3-transporter
HMMTigr	IPR003020	72	1011	TIGR00834	HCO3- transporter

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	432	ALSAILFIYLATVTNAITFGGLL	454	SECONDARY	23
	2	472	AVSGAIFCLFAGQPLTILSSTGP	494	SECONDARY	23
	3	522	LWSAFLCLILVATDASFLVQYFT	544	PRIMARY	23
	4	660	FILFLGTYTSSMALKKFKTSPYF	682	SECONDARY	23
	5	690	ISDFAIILSILIFCVIDALVGVD	712	PRIMARY	23
	6	741	WWVCLAAAIPALLVTILIFMDQQ	763	PRIMARY	23
	7	789	VAILMVICSLMALPWYVAATVIS	811	PRIMARY	23
	8	851	GLSVFMAPILKFIPMPVLYGVFL	873	PRIMARY	23
	9	905	LRHVPLRRVHLFTFLQVLCLALL	927	SECONDARY	23
	10	929	ILKSTVAAIIFPVMILALVAV	949	PRIMARY	21