ORK00273

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK00273

Order Kazusa clone(s) from :

Accession No
Product ID	ORK00273
Clone name	fg04050
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	SEMA4C
cDNA sequence	DNA sequence (6044 bp) Predicted protein sequence (934 aa)
HaloTag ORF Clone	FHC00273
Flexi ORF Clone	FXC00273
Description	Semaphorin-4C precursor.

Features of the cloned cDNA sequence

Description

Length: 6044 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : YES

Integrity of 3' end

Length of 3'UTR	889 bp
Genome contig ID	gi89161199r_96789201
PolyA signal sequence (AATAAA,-23)	+--------+--------+----*----+---- TTTATATTGACAAATAAAATGGAGAAAATGAAACG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ATTGCTCTGATGGGGCTAAGATGTTTGGGCTAAAGCCTTTCTGGGTACCA

Features of the protein sequence

Description

Length: 934 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q9C0C4	100.0	Semaphorin-4C; ...	Homo sapiens
AAQ89201	99.8	SEMA4C [Homo sa...	Homo sapiens
XP_001100467	97.2	semaphorin 4C [...	Macaca mulatta
EAW71325	94.9	sema domain, im...	Homo sapiens
XP_604387	86.8	similar to sema...	Bos taurus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR001627	154	582	PF01403	Semaphorin/CD100 antigen
HMMPfam	IPR002165	600	652	PF01437	Plexin
HMMSmart	IPR001627	154	582	SM00630	Semaphorin/CD100 antigen
	IPR003659	600	652	SM00423	Plexin/semaphorin/integrin
	IPR003599	663	747	SM00409	Immunoglobulin subtype
ProfileScan	IPR001627	131	598	PS51004	Semaphorin/CD100 antigen
ProfileScan	IPR007110	657	738	PS50835	Immunoglobulin-like

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	701	PGSFLYDARLQALVVMAA	718	SECONDARY	18
	2	736	RLAAEGYLVAVVAGPSVTLEAR	757	SECONDARY	22
	3	762	NLGLVWLAVVALGAVCLVLLLLV	784	PRIMARY	23