ORK01281

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK01281

Order Kazusa clone(s) from :

Accession No
Product ID	ORK01281
Clone name	sh03843
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	TAP2
cDNA sequence	DNA sequence (5448 bp) Predicted protein sequence (704 aa)
HaloTag ORF Clone	FHC01281
Flexi ORF Clone	FXC01281
Description	transporter 2, ATP-binding cassette, sub-family B (MDR/TAP)

Features of the cloned cDNA sequence

Description

Length: 5448 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : YES

Integrity of 3' end

Length of 3'UTR	3267 bp
Genome contig ID	gi89161210r_32801176
PolyA signal sequence (ATTAAA,-24)	+--------+--------+----*----+---- TTATTTTTTAAATTAAAAATATATGGCTATGTAGT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ATTCTATTTTATGGAAGTTCCATATTTCATTTATCCTGTTTCCTTCTACT

Features of the protein sequence

Description

Length: 704 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAD92190	100.0	transporter 2, ...	Homo sapiens
CAA80522	100.0	TAP2B [Homo sap...	Homo sapiens
AAA59841	99.8	putative peptid...	Homo sapiens
BAB71769	99.8	ABC-transporter...	Homo sapiens
AAB36588	99.5	ABC-transporter...	Gorilla gorilla

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR003439	607	648	PD000006	ABC transporter related
FPrintScan	IPR013306	38	52	PR01897	ABC transporter
	IPR013305	47	69	PR01896	ABC transporter
	IPR013306	53	67	PR01897	ABC transporter
	IPR013306	70	84	PR01897	ABC transporter
	IPR013306	102	116	PR01897	ABC transporter
	IPR013306	117	132	PR01897	ABC transporter
	IPR013306	179	193	PR01897	ABC transporter
	IPR013306	304	322	PR01897	ABC transporter
	IPR013306	357	371	PR01897	ABC transporter
	IPR013305	517	531	PR01896	ABC transporter
	IPR013306	573	588	PR01897	ABC transporter
	IPR013305	646	664	PR01896	ABC transporter
HMMPfam	IPR001140	153	424	PF00664	ABC transporter
HMMPfam	IPR003439	497	679	PF00005	ABC transporter related
HMMSmart	IPR003593	496	679	SM00382	AAA+ ATPase
HMMTigr	IPR005293	2	698	TIGR00958	Antigen peptide transporter 2
ProfileScan	IPR011527	154	436	PS50929	ABC transporter
ProfileScan	IPR003439	469	703	PS50893	ABC transporter related
ScanRegExp	IPR003439	608	622	PS00211	ABC transporter related

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	9	PWTSLLLVDAALLWLLQGPLGTL	31	SECONDARY	23
	2	60	LGFVGTLLLPLCLATPLTVSLRA	82	PRIMARY	23
	3	101	SWLLVGYGAAGLSWSLWAVLSPP	123	SECONDARY	23
	4	143	LKLSRPDLPLLVAAFFFLVLAVL	165	PRIMARY	23
	5	185	FDPHAFASAIFFMCLFSFGSSL	206	SECONDARY	22