ORK01647

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK01647

Order Kazusa clone(s) from :

Accession No	AB210040
Product ID	ORK01647
Clone name	pf00322
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	ABCA4
cDNA sequence	DNA sequence (7309 bp) Predicted protein sequence (2303 aa)
HaloTag ORF Clone	FHC01647
Flexi ORF Clone	FXC01647
Description	Retinal-specific ATP-binding cassette transporter (ATP-binding cassette sub-family A member 4) (RIM ABC transporter) (RIM protein) (RmP) (Stargardt disease protein).

Features of the cloned cDNA sequence

Description

Length: 7309 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	397 bp
Genome contig ID	gi89161185r_94130984
PolyA signal sequence (AATAAA,-25)	+--------+--------+----*----+---- TTCATGGAAAAATAAAATGCAAATGCACTCATCAC
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAACTATCCTAATTCACAGTCTCCCTGGTGTGCACCACCTAGTATAGTTT

Features of the protein sequence

Description

Length: 2303 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAE06122	100.0	ABCA4 variant p...	Homo sapiens
XP_001152577	99.1	ATP-binding cas...	Pan troglodytes
BAG10254	100.0	ATP-binding cas...	synthetic construct
P78363	99.9	Retinal-specifi...	Homo sapiens
AAC05632	99.8	rim ABC transpo...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR003439	1092	1134	PD000006	ABC transporter related
BlastProDom	IPR003439	2100	2143	PD000006	ABC transporter related
HMMPfam	IPR003439	986	1166	PF00005	ABC transporter related
HMMPfam	IPR003439	1995	2176	PF00005	ABC transporter related
HMMSmart	IPR003593	985	1175	SM00382	AAA+ ATPase
HMMSmart	IPR003593	1994	2178	SM00382	AAA+ ATPase
HMMTigr	IPR005951	31	2302	TIGR01257	Rim ABC transporter
ProfileScan	IPR003439	959	1190	PS50893	ABC transporter related
ProfileScan	IPR003439	1968	2200	PS50893	ABC transporter related
ScanRegExp	IPR003439	1092	1106	PS00211	ABC transporter related

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	1	GARGALNGVYVLCCLRGLSS	20	SECONDARY	20
	2	26	RVVISMGFVRQIQLLLWKNW	45	SECONDARY	20
	3	54	RFVVELVWPLSLFLVLIWLRNAN	76	PRIMARY	23
	4	681	LNRCFPIFMVLAWIYSVSMTVKS	703	SECONDARY	23
	5	725	VIWCTWFLDSFSIMSMSIFLLTI	747	PRIMARY	23
	6	759	DPFILFLFLLAFSTATIMLCFLL	781	PRIMARY	23
	7	791	AAACSGVIYFTLYLPHILCFAWQ	813	SECONDARY	23
	8	865	SFLLSMQMMLLDAAVYGLLAWYL	887	SECONDARY	23
	9	1403	FLAQIVLPATFVFLALMLSIVIP	1425	PRIMARY	23
	10	1705	TTSVDAVVAICVIFSMSFVPASF	1727	PRIMARY	23
	11	1759	LWDIMNYSVSAGLVVGIFIGFQ	1780	SECONDARY	22
	12	1791	PALVALLLLYGWAVIPMMYPASF	1813	PRIMARY	23
	13	1824	ALSCANLFIGINSSAITFILELF	1846	SECONDARY	23
	14	1910	FAMVVEGVVYFLLTLLVQRHFFL	1932	SECONDARY	23