ORK01880

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK01880

Order Kazusa clone(s) from :

Accession No
Product ID	ORK01880
Clone name	hk02176
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	TRPV1
cDNA sequence	DNA sequence (4149 bp) Predicted protein sequence (861 aa)
HaloTag ORF Clone	FHC01880
Flexi ORF Clone	FXC01880
Description	Transient receptor potential cation channel subfamily V member 1 (TrpV1) (osm-9-like TRP channel 1) (OTRPC1) (Vanilloid receptor 1) (Capsaicin receptor).

Features of the cloned cDNA sequence

Description

Length: 4149 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : YES

Integrity of 3' end

Length of 3'UTR	1365 bp
Genome contig ID	gi51511734r_3315493
PolyA signal sequence (AATAAA,-21)	+--------+--------+----*----+---- TACTGGCATCCTCAAATAAACAGATATGTATACAT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ATATATCGTGTCAAGTGTAAAGTTTGTGGCAGGAAATGGGCTGGCATAGC

Features of the protein sequence

Description

Length: 861 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAG11073	100.0	transient recep...	synthetic construct
ABA06605	99.7	transient recep...	Homo sapiens
Q8NER1	88.6	Transient recep...	Homo sapiens
CAB95729	88.5	vanilloid recep...	Homo sapiens
NP_542435	88.5	transient recep...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR008347	164	191	PR01768	Vanilloid receptor
	IPR008347	193	214	PR01768	Vanilloid receptor
	IPR002110	224	236	PR01415	Ankyrin
	IPR008347	233	251	PR01768	Vanilloid receptor
	IPR002110	236	248	PR01415	Ankyrin
	IPR008347	281	298	PR01768	Vanilloid receptor
	IPR008347	319	342	PR01768	Vanilloid receptor
	IPR008347	428	448	PR01768	Vanilloid receptor
	IPR008347	471	487	PR01768	Vanilloid receptor
	IPR008347	658	681	PR01768	Vanilloid receptor
	IPR008347	746	766	PR01768	Vanilloid receptor
	IPR008347	767	786	PR01768	Vanilloid receptor
HMMPfam	IPR002110	223	255	PF00023	Ankyrin
	IPR002110	270	305	PF00023	Ankyrin
	IPR002110	355	378	PF00023	Ankyrin
	IPR005821	553	705	PF00520	Ion transport
HMMSmart	IPR002110	176	208	SM00248	Ankyrin
	IPR002110	223	252	SM00248	Ankyrin
	IPR002110	270	302	SM00248	Ankyrin
	IPR002110	355	385	SM00248	Ankyrin
HMMTigr	IPR004729	119	847	TIGR00870	Transient receptor potential channel
ProfileScan	IPR002110	223	255	PS50088	Ankyrin
ProfileScan	IPR002110	223	378	PS50297	Ankyrin

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	456	IFYFNFLVYCLYMIIFTMAAYYR	478	PRIMARY	23
	2	496	RVTGEILSVLGGVYFFFRGIQYF	518	SECONDARY	23
	3	535	EMLFFLQSLFMLATVVLYFSHLK	557	PRIMARY	23
	4	566	SLALGWTNMLYYTRGFQQMGIYA	588	SECONDARY	23
	5	601	RFMFVYIVFLFGFSTAVVTLIED	623	PRIMARY	23
	6	683	IILLLAYVILTYILLLNMLIALM	705	PRIMARY	23