ORK02198

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK02198

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Accession No
Product ID	ORK02198
Clone name	bm05940
Vector information	The cDNA fragment was inserted at the XhoI-SacI site of the ... The cDNA fragment was inserted at the XhoI-SacI site of the pBC SK+ vector (Cm resistance; http://www.kazusa.or.jp/huge/vectorinfo/pbcskp_s.txt) with sequences including attB1 and attB2 sites (CTCGAGCAGCTGAAGCTTTCACAAGTTTGTAC AAAAAAGCAGGCTCTTC-5' end of cDNA, GAGCTCTCACCACTTTGTAC AAGAAAGCTGGGCGGCCGCTTTTTTTTTTTTTTTTTT-3' end of cDNA).
Symbol	APLP1
cDNA sequence	DNA sequence (2387 bp) Predicted protein sequence (656 aa)
HaloTag ORF Clone	FHC02198
Flexi ORF Clone	FXC02198
Description	Amyloid-like protein 1 precursor (APLP) (APLP-1) [Contains: C30].

Features of the cloned cDNA sequence

Description

Length: 2387 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	350 bp
Genome contig ID	gi42406306f_40951295
PolyA signal sequence (AATAAA,-21)	+--------+--------+----*----+---- CCTACTAACATCCCAATAAAGTCCTCTTCCCTACC
Flanking genome sequence (111240 - 111289)	----+--------+--------+--------+--------+----* AGGCCAGTCTGAGTCTCTGTGGGAGAATTAGTCTGTAACCACAGCTGGAC

Features of the protein sequence

Description

Length: 656 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
P51693	100.0	Amyloid-like pr...	Homo sapiens
AAB96331	99.8	amyloid precurs...	Homo sapiens
BAG35219	99.8	unnamed protein...	Homo sapiens
NP_001019978	99.8	amyloid precurs...	Homo sapiens
BAE01947	98.0	unnamed protein...	Macaca fascicularis

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR008155	206	224	PR00203	Amyloidogenic glycoprotein
	IPR008155	307	330	PR00203	Amyloidogenic glycoprotein
	IPR008155	585	610	PR00203	Amyloidogenic glycoprotein
	IPR008155	632	654	PR00203	Amyloidogenic glycoprotein
HMMPfam	IPR008154	52	217	PF02177	Amyloidogenic glycoprotein
HMMSmart	IPR008154	52	217	SM00006	Amyloidogenic glycoprotein
ScanRegExp	IPR008155	210	217	PS00319	Amyloidogenic glycoprotein
ScanRegExp	IPR008155	643	650	PS00320	Amyloidogenic glycoprotein

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	25	PLPLLLPLLLLLLRAQPAIGSLA	47	SECONDARY	23
SOSUI2	2	588	VSGLLIMGAGGGSLIVLSMLLL	609	PRIMARY	22