ORK04012

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK04012

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Accession No	AB209084
Product ID	ORK04012
Clone name	hk05319
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	ABCC8
cDNA sequence	DNA sequence (4190 bp) Predicted protein sequence (1011 aa)
Description	ATP-binding cassette transporter sub-family C member 8 (Sulfonylurea receptor 1).

Features of the cloned cDNA sequence

Description

Length: 4190 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : YES

Integrity of 3' end

Length of 3'UTR	1153 bp
Genome contig ID	gi51511727r_17275039
PolyA signal sequence (None)	+--------+--------+----*----+---- CCCTCATCTCCCCTGGACAGAAGATCGGGATCTGC
Flanking genome sequence (279904 - 279855)	----+--------+--------+--------+--------+----* ACCCAGACACCCCATGTTGCTGCACTCGGGTCACTGCAGAGTTCAGAAAG

Features of the protein sequence

Description

Length: 1011 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAD92321	100.0	Sulfonylurea re...	Homo sapiens
XP_001366704	87.1	similar to ATP-...	Monodelphis dom...
XP_421005	80.6	similar to sulf...	Gallus gallus
XP_001232387	80.1	similar to sulf...	Gallus gallus
XP_001149494	67.6	ATP-binding cas...	Pan troglodytes

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR003439	832	874	PD000006	ABC transporter related
FPrintScan	IPR000844	23	33	PR01093	Sulphonylurea receptor
	IPR000388	39	66	PR01092	Sulphonylurea receptor
	IPR000388	79	93	PR01092	Sulphonylurea receptor
	IPR000388	136	150	PR01092	Sulphonylurea receptor
	IPR000388	218	241	PR01092	Sulphonylurea receptor
	IPR000844	283	291	PR01093	Sulphonylurea receptor
	IPR000388	371	393	PR01092	Sulphonylurea receptor
	IPR000844	621	633	PR01093	Sulphonylurea receptor
	IPR000844	642	658	PR01093	Sulphonylurea receptor
HMMPfam	IPR001140	332	604	PF00664	ABC transporter
HMMPfam	IPR003439	710	909	PF00005	ABC transporter related
HMMSmart	IPR003593	709	917	SM00382	AAA+ ATPase
ProfileScan	IPR011527	314	616	PS50929	ABC transporter
ProfileScan	IPR003439	683	933	PS50893	ABC transporter related
ScanRegExp	IPR003439	833	847	PS00211	ABC transporter related

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	41	FVDALNVVPHVFLLFITFPILFI	63	SECONDARY	23
	2	89	WILTFMLLFVLVCEIAEGILSDG	111	PRIMARY	23
	3	118	LHLYMPAGMAFMAAVTSVVYYH	139	SECONDARY	22
	4	145	NFPKLLIALLVYWTLAFITKTIK	167	PRIMARY	23
	5	183	FCLTGLLVILYGMLLLVEVNVIR	205	PRIMARY	23
	6	230	RFLQPFVNLLSKGTYWWMNAFIK	252	SECONDARY	23
	7	317	STFRILADLLGFAGPLCIFGIVD	339	SECONDARY	23
	8	365	FLANAYVLAVLLFLALLLQRTFL	387	SECONDARY	23
	9	443	MWFFFLCPNLWAMPVQIIVGVIL	465	PRIMARY	23
	10	470	LGVSALIGAAVIILLAPVQYFVA	492	PRIMARY	23
	11	551	AIYTSISIFMNTAIPIAAVLITF	573	PRIMARY	23
	12	594	LSLFHILVTPLFLLSSVVRSTVK	616	SECONDARY	23