ORK09882

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK09882

Order Kazusa clone(s) from :

Accession No
Product ID	ORK09882
Clone name	bm04184
Vector information	The cDNA fragment was inserted at the XhoI-SacI site of the ... The cDNA fragment was inserted at the XhoI-SacI site of the pBC SK+ vector (Cm resistance; http://www.kazusa.or.jp/huge/vectorinfo/pbcskp_s.txt) with sequences including attB1 and attB2 sites (CTCGAGCAGCTGAAGCTTTCACAAGTTTGTAC AAAAAAGCAGGCTCTTC-5' end of cDNA, GAGCTCTCACCACTTTGTAC AAGAAAGCTGGGCGGCCGCTTTTTTTTTTTTTTTTTT-3' end of cDNA).
Symbol	PSEN2
cDNA sequence	DNA sequence (2070 bp) Predicted protein sequence (476 aa)
HaloTag ORF Clone	FHC09882
Flexi ORF Clone	FXC09882
Description	presenilin 2 (Alzheimer disease 4)

Features of the cloned cDNA sequence

Description

Length: 2070 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	517 bp
Genome contig ID	gi89161185f_225029689
PolyA signal sequence (AATAAA,-23)	+--------+--------+----*----+---- AATAGTAATATCAATAAATAGATGAGTCCTGTTAG
Flanking genome sequence (120733 - 120782)	----+--------+--------+--------+--------+----* AATCTTGGAGTTTGGTCCGTTGTAAATGTTGACCCCTCTCCCTGCATCTT

Features of the protein sequence

Description

Length: 476 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
XP_001087264	8.4e-186	97.6	presenilin 2 [M...	Macaca mulatta
CAH73111	2.7e-183	100.0	presenilin 2 (A...	Homo sapiens
EAW69796	3.7e-182	99.7	presenilin 2 (A...	Homo sapiens
P49810	1.8e-180	100.0	Presenilin-2; S...	Homo sapiens
AAP36366	1.8e-180	100.0	presenilin 2 (A...	synthetic construct

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001493	32	54	PR01074	Peptidase A22A
	IPR001493	65	76	PR01074	Peptidase A22A
	IPR001108	120	130	PR01072	Peptidase A22A
	IPR001108	274	281	PR01072	Peptidase A22A
	IPR001108	291	308	PR01072	Peptidase A22A
	IPR001108	317	324	PR01072	Peptidase A22A
	IPR001493	334	352	PR01074	Peptidase A22A
	IPR001493	355	371	PR01074	Peptidase A22A
HMMPfam	IPR001108	104	467	PF01080	Peptidase A22A
HMMSmart	IPR006639	164	462	SM00730	Peptidase A22

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	114	KHVIMLFVPVTLCMIVVVATIKS	136	PRIMARY	23
	2	166	SVLNTLIMISVIVVMTIFLVVLY	188	PRIMARY	23
	3	195	FIHGWLIMSSLMLLFLFTYIYLG	217	PRIMARY	23
	4	226	AMDYPTLLLTVWNFGAVGMVCIH	248	SECONDARY	23
	5	266	LMALVFIKYLPEWSAWVILGAIS	288	PRIMARY	23
	6	313	NEPIFPALIYSSAMVWTVGMAKL	335	SECONDARY	23
	7	388	VKLGLGDFIFYSVLVGKAAATG	409	SECONDARY	22
	8	425	IGLCLTLLLLAVFKKALPALPIS	447	PRIMARY	23