ORK11524

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK11524

Order Kazusa clone(s) from :

Accession No
Product ID	ORK11524
Clone name	fh19224
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	GABRB3
cDNA sequence	DNA sequence (5677 bp) Predicted protein sequence (477 aa)
HaloTag ORF Clone	FHC11524
Flexi ORF Clone	FXC11524
Description	gamma-aminobutyric acid (GABA) A receptor, beta 3

Features of the cloned cDNA sequence

Description

Length: 5677 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	4242 bp
Genome contig ID	gi51511731r_24239793
PolyA signal sequence (ATTAAA,-19)	+--------+--------+----*----+---- GAATGAATTCAAATTTATTAAAAAAGGAGTAACTG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ACCTAAATGTGAAGACTGATTTTTTTTTCTTCCTTGGCTATCTTGTGAAG

Features of the protein sequence

Description

Length: 477 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
P28472	1.8e-211	100.0	Gamma-aminobuty...	Homo sapiens
XP_001109118	3.9e-211	99.5	gamma-aminobuty...	Macaca mulatta
AAH10641	7.4e-211	99.7	Gamma-aminobuty...	Homo sapiens
XP_510254	3.2e-210	99.3	gamma-aminobuty...	Pan troglodytes
P63079	1.2e-207	97.2	Gamma-aminobuty...	Rattus norvegicus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR006201	87	103	PR00252	Neurotransmitter-gated ion-channel
	IPR002289	100	114	PR01160	Gamma-aminobutyric-acid A receptor
	IPR006201	119	130	PR00252	Neurotransmitter-gated ion-channel
	IPR006201	165	179	PR00252	Neurotransmitter-gated ion-channel
	IPR002289	204	217	PR01160	Gamma-aminobutyric-acid A receptor
	IPR006201	241	253	PR00252	Neurotransmitter-gated ion-channel
	IPR006028	250	270	PR00253	Gamma-aminobutyric acid A receptor
	IPR006028	276	297	PR00253	Gamma-aminobutyric acid A receptor
	IPR002289	304	316	PR01160	Gamma-aminobutyric-acid A receptor
	IPR006028	310	331	PR00253	Gamma-aminobutyric acid A receptor
	IPR002289	443	457	PR01160	Gamma-aminobutyric-acid A receptor
	IPR006028	455	475	PR00253	Gamma-aminobutyric acid A receptor
HMMPfam	IPR006202	41	247	PF02931	Neurotransmitter-gated ion-channel ligand-binding
HMMPfam	IPR006029	254	472	PF02932	Neurotransmitter-gated ion-channel transmembrane region
HMMTigr	IPR006201	5	475	TIGR00860	Neurotransmitter-gated ion-channel
ScanRegExp	IPR006201	165	179	PS00236	Neurotransmitter-gated ion-channel

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	10	GGRLFGIFSAPVLVAVVCCAQSV	32	PRIMARY	23
	2	248	GYFILQTYMPSILITILSWVSFW	270	PRIMARY	23
	3	313	YLMGCFVFVFLALLEYAFVNYIF	335	PRIMARY	23