ORK06041

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK06041

Order Kazusa clone(s) from :

Accession No	AB209886
Product ID	ORK06041
Clone name	eg00416
Vector information	The cDNA fragment was inserted at the XhoI-SacI site of the ... The cDNA fragment was inserted at the XhoI-SacI site of the pBC SK+ vector (Cm resistance; http://www.kazusa.or.jp/huge/vectorinfo/pbcskp_s.txt) with sequences including attB1 and attB2 sites (CTCGAGCAGCTGAAGCTTTCACAAGTTTGTAC AAAAAAGCAGGCTCTTC-5' end of cDNA, GAGCTCTCACCACTTTGTAC AAGAAAGCTGGGCGGCCGCTTTTTTTTTTTTTTTTTT-3' end of cDNA).
Symbol	MSH5
cDNA sequence	DNA sequence (6486 bp) Predicted protein sequence (489 aa)
Description	MutS protein homolog 5.

Features of the cloned cDNA sequence

Description

Length: 6486 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	142 bp
Genome contig ID	gi89161210f_31725558
PolyA signal sequence (AATAAA,-19)	+--------+--------+----*----+---- TTTGTTTCATATTAGGAATAAAGTTTATTTTGAAG
Flanking genome sequence (112873 - 112922)	----+--------+--------+--------+--------+----* AAAGATATTGTTTCTTTAGTCTCAAAACAAGAGACTAGGAAAGATCCAAA

Features of the protein sequence

Description

Length: 489 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAD93123	4.3e-205	100.0	MSH5 protein va...	Homo sapiens
XP_001158071	5.9e-198	99.5	mutS homolog 5 ...	Pan troglodytes
CAI17726	4.9e-193	99.5	mutS homolog 5 ...	Homo sapiens
CAQ07247	6.7e-193	100.0	mutS homolog 5 ...	Homo sapiens
AAH41031	3.1e-192	99.3	MSH5 protein [H...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR000432	325	431	PD001263	DNA mismatch repair protein MutS
HMMPfam	IPR007861	83	181	PF05190	MutS IV
HMMPfam	IPR000432	229	449	PF00488	DNA mismatch repair protein MutS
HMMSmart	IPR007696	4	254	SM00533	MutS III
HMMSmart	IPR000432	270	447	SM00534	DNA mismatch repair protein MutS
ScanRegExp	IPR000432	351	367	PS00486	DNA mismatch repair protein MutS

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	3	PYHHLNPSWMWPVLLFVFLSQTV	25	SECONDARY	23
	2	116	RIPSCSVIYIPLIGFLLSIPRLP	138	PRIMARY	23
	3	200	RAAVLTRVLDLASRLDVLLALAS	222	SECONDARY	23
	4	284	SIYLKQVGLITFMALVGSFVPAE	306	PRIMARY	23