ORK05333

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK05333

Order Kazusa clone(s) from :

Accession No	AB209525
Product ID	ORK05333
Clone name	fk09872
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	GPM6B
cDNA sequence	DNA sequence (2780 bp) Predicted protein sequence (280 aa)
Description	Neuronal membrane glycoprotein M6-b (M6b).

Features of the cloned cDNA sequence

Description

Length: 2780 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	1937 bp
Genome contig ID	gi89161218r_13598984
PolyA signal sequence (AATAAA,-17)	+--------+--------+----*----+---- ATGTACTAAGAGTAATGAAATAAAATTATAGCTTC
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ATTCACTTGCCTTTTTACCATACATAAATCCTATCTTTCTTTGTTCTCAG

Features of the protein sequence

Description

Length: 280 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAD92762	1.2e-124	100.0	glycoprotein M6...	Homo sapiens
CAH93501	5.6e-119	100.0	hypothetical pr...	Pongo abelii
EAW98842	6.1e-119	96.4	glycoprotein M6...	Homo sapiens
BAC04600	6.4e-119	96.4	unnamed protein...	Homo sapiens
CAH91404	1.7e-118	96.1	hypothetical pr...	Pongo abelii

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001614	17	46	PR00214	Myelin proteolipid protein PLP
	IPR001614	71	99	PR00214	Myelin proteolipid protein PLP
	IPR001614	101	130	PR00214	Myelin proteolipid protein PLP
HMMPfam	IPR001614	13	257	PF01275	Myelin proteolipid protein PLP
HMMSmart	IPR001614	155	215	SM00002	Myelin proteolipid protein PLP
ScanRegExp	IPR001614	39	48	PS00575	Myelin proteolipid protein PLP
ScanRegExp	IPR001614	205	224	PS01004	Myelin proteolipid protein PLP

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	11	AMGCFECCIKCLGGVPYASLVAT	33	SECONDARY	23
	2	40	VALFCGCGHVALAGTVAILEQHF	62	SECONDARY	23
	3	75	VIQLMQYVIYGIASFFFLYGIIL	97	PRIMARY	23
	4	126	GMFVFLTYVLGVAWLGVFGFSAV	148	PRIMARY	23
	5	216	LFIVACAGAGATVIALIHFLMIL	238	PRIMARY	23